A2780


A2780
Cell Line Name
Cell Line Description
The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (ECACC catalogue no. 93112517) and the adriamycin resistant cell line A2780 ADR (ECACC catalogue no. 93112520).
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General Info
Species
Human
Links
Cellosaurus: CVCL_0134
Release Conditions
Characteristics
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X
CSF1PO: 10,11
D5S818: 11,12
D7S820: 10
D13S317: 12,13
D16S539: 11,13
TH01: 6
TPOX: 8,10
vWA: 15,16
Karyotype
Applications
Toxicity testing, cancer genetic studies
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Semin Oncol 1984;11:285; Cancer Res 1987;47:414
Bibliography
Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.
Documents
- 93112519_A2780_Growth Profile
- Analysis of Drug Resistant Properties of A2780 Ovarian Cancer Cell Lines Using Label-Free Automated Microscopy (IncuCyte ZOOM)
- Passage numbers explained
- MSDS for nucleic acids
- MSDS For Frozen Cell Culture
- CRT Cell Line Release Authorisation for Research Use in Commercial Organisations
- Important technical information for successful culture of your cells
Images
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: A2780 (ECACC 93112519).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.